Mouse Anti-ERBB2 Recombinant Antibody (clone ABT-Her2)
CAT#: ZG-0100C
This product is a mouse antibody that recognizes ERBB2. It can be used in a wide variety of applications, such as: IHC-P, IF.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min)
IHC
Figure 2 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 3 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 4 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 5 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min)
IHC
Figure 6 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 7 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 8 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 9 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 10 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 11 Mouse Anti-ERBB2 Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Breast Carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Normal Tissue
Figure 3 High expression in esophagus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Normal Tissue
Figure 7 Breast
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
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Specifications
- Immunogen
- Synthesized peptide derived from human Her-2(c-erbB-2)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Her-2(c-erbB-2)
- Species Reactivity
- Human, Mouse
- Clone
- ABT-Her2
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.
Target
- Alternative Names
- Erb-B2 Receptor Tyrosine Kinase 2; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; HER2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog)
- Gene ID
- 2064
- UniProt ID
- P04626
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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RELATED PRODUCTS
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Customer Review
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Positive Lab Feedback
The responses from everyone in the lab have been overwhelmingly positive. All agree that this antibody greatly raises the caliber and dependability of our studies.
Great Technical Support
The technical support staff answered all questions in-depth and quickly. Their help has been crucial to making sure our experiments go off without a hitch.
Minimal Background Noise
Minimal background noise was seen during the assays, which greatly increased the experiment's signal-to-noise ratio and produced more accurate data.
Q&As
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What is the importance of antibody validation?
A: By ensuring that the antibody attaches to the target antigen specifically and functions properly in the circumstances of your particular application, validation guarantees consistent and dependable results.
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Can I reuse primary antibodies in my experiments?
A: Reusing primary antibodies can decrease binding effectiveness and increase background noise because of possible carryover, even though it is possible. For optimal effects, use fresh aliquots.
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How do I store reconstituted antibody solutions?
A: To prevent freeze-thaw cycles, store reconstituted solutions in aliquots at -20°C or lower. Certain antibodies can also be kept for brief periods of time-roughly one month-at 4°C.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0100C, RRID: AB_3112062)
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Related Signaling Pathways
ErbB Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
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|---|---|---|---|
| TAB-040CT | Anti-Human HER2/neu Recombinant Antibody (C6.5) | ELISA, WB | Human antibody |
| TAB-042CT | Anti-Human HER2/neu Recombinant Antibody (CRX01) | WB | Human antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-033CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-033CT-F(E)) | ELISA, WB | Humanized Fab |
| TAB-038CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-038CT-F(E)) | WB | Humanized Fab |
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| TAB-041CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-041CT-F(E)) | ELISA, WB | Humanized Fab |
| TAB-050CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (HuA21) | ELISA, WB | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-148LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-014LC | Mouse Anti-ERBB2 Recombinant Antibody (MOB-014LC) | ELISA, FC | Mouse IgG |
| MOB-014LC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (MOB-014LC-F(E)) | ELISA, FC | Mouse Fab |
| MOB-014LC-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (MOB-014LC-S(P)) | ELISA, FC | Mouse scFv |
| MRO-1926-CN | Rabbit Anti-ErbB2 Polyclonal Antibody (MRO-1926-CN) | WB, IF, IHC | Rabbit IgG |
| MRO-1927-CN | Rabbit Anti-ErbB2 Polyclonal Antibody (MRO-1927-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-FY-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-0010-FY-S(P)) | ELISA, FC | Human scFv |
| HPAB-0011-FY-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-0011-FY-S(P)) | Inhib | Mouse scFv |
| PSBC-447 | Human Anti-ERBB2 Recombinant Antibody (clone 39S); scFv Fragment | ELISA, FuncS | Human scFv |
| HPAB-0193-WJ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone 6H8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-M0073-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-M0073-YC-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0608LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0608LY-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0609LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0609LY-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0610LY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0610LY-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0694-FY-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone VL311); Fab Fragment | ELISA, Activ | Humanized Fab |
| HPAB-0695-FY-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone BW835); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-468CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-053) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-761) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-005) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY91 | AbPlus™ Anti-ERBB2 Magnetic Beads (3.F2) | IP, Protein Purification | |
| VS-0724-YC1516 | AbPlus™ Anti-Erbb2 Magnetic Beads (VS-0724-YC1516) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY24 | Human Anti-ERBB2 (clone 1.18.1) scFv-Fc Chimera | FC, BI | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY108 | CytoStream™ Mouse Anti-ERBB2 Recombinant Antibody (clone 24D2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC430 | Recombinant Anti-ERBB2 Vesicular Antibody, EV Displayed (VS-0425-YC430) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2335 | Anti-ERBB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2336 | Anti-Mouse ERBB2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC3 | Recombinant Anti-ERBB2 Biparatopic Antibody, Symmetric scFv-Fab | ELISA | Symmetric scFv-Fab |
| VS-0525-YC6 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero Fab | ELISA | Asymmetric hetero Fab |
| VS-0525-YC7 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero HC-LC | ELISA, Inhib | Asymmetric hetero HC, common LC |
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